Proper Citation : Agilent Seahorse XFe96 (Agilent, America)

Description: Agilent Seahorse XF analysis uses label free technology to real-time detect discrete changes in cellular energy metabolism, which helps to understand the key functions driving cell signal transduction, proliferation, activation, toxicity, and biosynthesis.

Acknowledgement: We thank Wenying Long and Yao Wu from the Department of Core Facilities, the Fourth Affiliated Hospital of School of Medicine, and International School of Medicine, International Institutes of Medicine, Zhejiang University, for the technical support.

Technician: Wenying Long, Yao Wu

XF代谢分析系统采用无需标记的固态探针在微孔板上同时测量细胞内两条主要的能量通路：线粒体呼吸和糖酵解。线粒体消耗氧气，氧化脂肪酸或其他底物以生成ATP。XF代谢分析系统通过测量细胞氧消耗速率（OCR）以检测线粒体呼吸。对于糖酵解途径来说，细胞在没有氧气参与的情况下通过糖酵解生成ATP,以及乳酸和氢离子。XF代谢分析系统通过测量胞外酸化速率（ECAR）以检测糖酵解。XF适用于大多数细胞类型，包括原代细胞、贴壁细胞和悬浮细胞，胰岛，组织，线粒体及其他细胞器，酵母，线虫，斑马鱼胚胎等。海马能量分析仪可以用于各种科研领域，比如线粒体疾病，糖尿病肥胖研究，细胞衰老，转化医学，心血管疾病研究，肿瘤研究，毒理学，免疫学，药物筛选以及神经代谢疾病研究。